identification of mycobacterium tuberculosis complex, using molecular methods

نویسندگان

مریم ارم آبادی

m eramabadi ، دانشگاه ازاد اسلامی، تنکابن، ایران کیوان تدین

k tadayon ، ﻣﻮﺳﺴﻪ ﺗﺤﻘﯿﻘﺎت واﮐﺴﻦ و ﺳﺮم ﺳﺎزی رازی، ﮐﺮج، ایران نادر مصوری

n mosavari ﻣﻮﺳﺴﻪ ﺗﺤﻘﯿﻘﺎت واﮐﺴﻦ و ﺳﺮم ﺳﺎزی رازی، ﮐﺮج، ایران روح اله کشاورز

r keshavarz رضا بنی هاشمی

چکیده

abstract background and objective: a high level of homogeneity observed within all bacteria in the mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. the work presented here was conducted to characterize mycobacterium tuberculosis complex isolates in golestan, northern iran. material and methods: between 2008 and 2010, 42 mycobacterial isolates were collected from clinical tuberculosis-suspected patients in golestan province. the isolates were sub-cultured on fresh mycobacterium-specific culture media including glycerinated and pyruvated lowenstein-jensen slopes. the isolates were subsequently subjected to a pcr-based identification scheme coined huard-warren method. this strategy consisted of three individual algorithms namely, 16srrna rv typing (rv0577, rv3877.8, rv1970, rv3120, rv1510 and is1561) and rd typing (rd1, rd 4, rd9 and rd12). results: all isolates were proved to be m. tuberculosis. furthermore, none of the patients were being infected with any other member of the m. tuberculosis complex or simultaneously co-infected with two mycobacteria. this fundamental observation was independently obtained by specific culture media, rv typing and also rd typing. conclusion: considering the fact that cattle and sheep farming play an important role in the economy of the region, absence of mycobacterium bovis in the studied isolates can be unexpected to some extent. huard-warren which is a simple and cost-effective identification method can be used in both reference and regional laboratory for differential diagnosis of tuberculosis. keywords: mycobacterium tuberculosis complex, huard-warrenmethod, 16srrna, golestan province, rd typing, rv typing

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification of Mycobacterium Tuberculosis Complex, Using Molecular Methods

Abstract Background and Objective: A high level of homogeneity observed within all bacteria in the Mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. The work presented here was conducted to characterize Mycobacterium tuberculosis complex isolates in Golestan, Northern Iran. ...

متن کامل

Molecular Identification of Mycobacterium Tuberculosis Complex in Formalin-Fixed, Paraffin-Embedded Tissue Blocks of Extra Pulmonary Speciemens using Genomics Extraction

Background: Tuberculosis has been detected in some extra pulmonary ecological niches. Although extra pulmonary tuberculosis (EPTB) is less frequent than Pulmonary Tuberculosis (PTB), its incidence has increased worldwide. The aim of this study was to investigate the presence of EPTB and MDR-EXPT in Formalin-fixed, paraffin-embedded tissue blocks among different samples in Kerma...

متن کامل

Detection of Isoniazid-Resistant Clinical isolates of Mycobacterium tuberculosis from India using Ser315Thr marker by Comparison of molecular methods

In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA  sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...

متن کامل

Identification of Mycobacterium tuberculosis adherence-mediating components: a review of key methods to confirm adhesin function

Anti-adhesion therapy represents a potentially promising avenue for the treatment and prevention of tuberculosis in a post-antibiotic era. Adhesins are surface-exposed microbial structures or molecules that enable pathogenic organisms to adhere to host surfaces, a fundamental step towards host infection. Although several Mycobacterium tuberculosis adhesins have been identified, it is predicted ...

متن کامل

comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...

15 صفحه اول

prevalence of Mycobacterium tuberculosis in patients infected with HIV by microscopical and molecular methods

Background:In some countries, one of the first and most common manifestations of HIV positive patients is tuberculosis (TB). HIV positive people are prone to other infections such as tuberculosis due to immune deficiency and reduced CD4+ cell count. Although increasing access to antiretroviral therapy has led to a reduction in HIV-related opportunistic infections and mortality, the simultaneous...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical laboratory journal

جلد ۷، شماره ۵، صفحات ۹-۱۵

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023